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Objective@#To compare the early and mid-term postoperative changes of left ventricular structure and function beteen mitral repair and replacement in patients with mitral regurgitation.@*Methods@#100 patients with degenerative mitral regurgitation underwent mitral valve replacement and mitral repair from January 2008 to January 2018 were retrospectively studyed. Of them, 46 patients underwent mitral repair and(repair group) 54 patients underwent mitral valve replacement(replacement group). The results of color Doppler echocardiography before, one week after, 12 months after and 24-36 months after operation were collected. Left atrial diameter(LAD), left ventricular end diastolic diameter(LVEDD) and left ventricular end systolic diameter(LVESD) were selected to evaluate left ventricular structure, fraction shortening(FS)、left ventricular stroke volume( SV )and left ventricular ejection fraction(LVEF) to evaluate left ventricular function. The data were analyzed by SPSS 22.0.@*Results@#In left ventricular structural parameters, LAD, LVEDD and LVESD in mitral repair group and replacement group were significantly improved compared with those before operation(P<0.05). There was no significant difference in LAD, LVEDD and LVESD between the two groups at 12 months after operation(P>0.05). There were significant differences in LAD(42.26 mm vs 47.15 mm), LVEDD(52.97 mm vs 60.18 mm) and LVESD(31.34 mm vs 34.82 mm) between the two groups at 24-36 months of follow-up(P<0.05). Among the left ventricular function indicators, the early and mid-term SV of the two groups were significantly improved compared with that of the preoperative group(P<0.05). LVEF(0.64 vs 0.59、0.64 vs 0.58)was significantly improved in the 12 and 24-36 months after the operation, and FS(36.18% vs 31.47%) was significantly different in the 24-36 months after the operation(P<0.05).@*Conclusion@#Mitral repair has high technical requirements and long operation time, but it has obvious advantages over mitral valve replacement in maintaining left ventricular structure and function in the middle and late period after operation.
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Objective To compare the early and mid-term postoperative changes of left ventricular structure and function beteen mitral repair and replacement in patients with mitral regurgitation. Methods 100 patients with degenerative mitral re-gurgitation underwent mitral valve replacement and mitral repair from January 2008 to January 2018 were retrospectively stud-yed. Of them, 46 patients underwent mitral repair and(repair group) 54 patients underwent mitral valve replacement(replace-ment group) . The results of color Doppler echocardiography before, one week after, 12 months after and 24-36 months after operation were collected. Left atrial diameter( LAD) , left ventricular end diastolic diameter( LVEDD) and left ventricular end systolic diameter(LVESD) were selected to evaluate left ventricular structure, fraction shortening(FS)、left ventricular stroke volume( SV )and left ventricular ejection fraction(LVEF) to evaluate left ventricular function. The data were analyzed by SPSS 22. 0. Results In left ventricular structural parameters, LAD, LVEDD and LVESD in mitral repair group and replace-ment group were significantly improved compared with those before operation(P<0. 05). There was no significant difference in LAD, LVEDD and LVESD between the two groups at 12 months after operation(P>0. 05). There were significant differences in LAD(42. 26 mm vs 47. 15 mm), LVEDD(52. 97 mm vs 60. 18 mm) and LVESD(31. 34 mm vs 34. 82 mm) between the two groups at 24-36 months of follow-up(P<0. 05). Among the left ventricular function indicators, the early and mid-term SV of the two groups were significantly improved compared with that of the preoperative group(P<0. 05). LVEF(0. 64 vs 0. 59、0. 64 vs 0. 58)was significantly improved in the 12 and 24-36 months after the operation, and FS(36. 18％ vs 31. 47％) was significantly different in the 24-36 months after the operation(P<0. 05). Conclusion Mitral repair has high technical requirements and long operation time, but it has obvious advantages over mitral valve replacement in maintaining left ventricular structure and function in the middle and late period after operation.
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Objective To investigate the effects of autophagy on cell migration and apoptosis of endothelial progenitor cells (EPCs) in hypoxic environment.Methods Rats' bone marrow-derived EPCs were isolated by density-gradient centrifugation,identified with double-fluorescent staining method,and then divided into 4 groups:normoxia (hypoxia 0h),hypoxia 1h,hypoxia 3h and hypoxia 6h groups for detecting the effects of hypoxia on EPCs migration and apoptosis.Transwell chambers and flow cytometry were used to detect cell migration and apoptosis respectively,and Western blotting was performed to detect the changes of autophagy-related proteins,including microtubule associated protein 1 light chain 3 (LC3-Ⅱ / Ⅰ) and ubiquitin of p62.To investigate the effects of hypoxic-induced autophagy on EPCs migration and apoptosis,cells were divided into 4 groups:hypoxia 0h,hypoxia 3h,hypoxia 3h+ShAtg7 and ShAtg7 groups,and then to detect again the EPCs migration and apoptosis.Results Compared to hypoxia 0h group,the EPCs migration markedly reduced and apoptosis increased in hypoxic 3h and 6h groups with statistically significant difference (P＜0.05).Western blotting showed the expressions of LC3-Ⅱ / Ⅰ significantly increased and of p62 decreased in EPCs by hypoxic treatment for 3 and 6 hours (P＜0.05),implying the increases of autophagy level.After inhibiting autophagy by silencing Atg7,hypoxia 3h+ShAtg7 group further reduced EPCs migration (P＜0.05) and aggravated apoptosis (P＜0.05) compared with hypoxia 3h group.Conclusion Autophagy may play a cytoprotective role through promoting EPCs migration and decreasing EPCs apoptosis in hypoxic environment.
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Objective To investigate the relationship between the single nucleotide polymorphisms(SNP)of rs11706903,intron region of Atg7,and Parkinson's disease(PD).Methods From January,2013 to March,2017,130 PD patients and 109 healthy subjects were recruit-ed and collected the blood samples.SNPs of rs11706903 were detected with polymerase chain reaction restriction fragment length polymor-phism.Results For the patients,the genotype frequencies of rs11706903 were AA 10.00%,AC 52.31% and CC 37.69%;and allele frequen-cies were A 36.15% and C 63.85%.For the healthy controls,the genotype frequencies of rs11706903 were AA 7.34%,AC 49.54% and CC 43.12%;and allele frequencies were A 32.11% and C 67.89%.There was no significant difference in frequencies of genetypes and alleles be-teen two groups(χ 2<1.001,P>0.05).Conclusion There might be no relationship between SNPs of Atg7 intron region rs11706903 and PD.
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Objective:To investigate the cervical lymphatic metastasis rates of clinically negative neck lymph node (cN0) maxillary malignant tumors,to compare the cervical lymphatic metastasis rates of the various pathological types,and to provide the reference for the treatment of the neck of the patients with cN0 maxillary malignant tumor.Methods:The clinical data of 277 cases with cN0 maxillary malignant tumor,treated in the department of oral and maxillofacial surgery of Peking University School and Hospital of Stomatology from 1990 to 2010,were reviewed.The cervical lymph node metastasis and the related clinical information were recorded.The clinical information including histopathology type of the tumors,tumor grade,primary site and TNM staging,as well as other demographic and clinical data,were retrieved from the electronic medical record system (EMRS) of the hospital.The pathogenesis of cervical lymph node metastasis in maxillary malignant tumors of different histopathological types,and the factors related to lymph node metastasis of upper cervical malignancy were analyzed by SPSS 19.0 statistical software.Results:The overall cervical lymph node metastasis rate of the 277 patients with cN0 maxillary malignant tumor was 15.5％ (43/277).Maxillary squamous cell carcinoma (SCC) had a strong cervical lymph node metastasis tendency and the rate was 33.0％.The overall metastatic rate of adenocarcinoma was 7.6％ lower than that of SCC,and the occurrence of cervical lymph node metastasis time was relatively late,but the metastasis rate of highly malignant grade salivary gland carcinoma was significantly higher than that of intermediate and low grade carcinoma (P =0.037).The metastatic rates of some highly malignant cN0 salivary gland carcinomas including adenocarcinoma,not other specified,high-grade mucoepidermoid carcinoma (MEC),and salivary duct carcinoma were exceeded 15％,while the metastasis rates of adenoid cystic carcinoma and myoepithelial carcinoma were lower.The metastasis rate of the sarcomas was very low with the rate of 4.9％.Conclusion:Selective neck dissection (SND) is recommended for cN0 maxillary SCC and feasible for some highly malignant cN0 salivary gland carcinomas including adenocarcinoma,not other specified,high-grade MEC,salivary duct carcinoma.The neck can be closely observed for the patients with maxillary sarcoma.
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AIM: To investigate the changes of serum angiogenic factors including vascular endothelial growth factor (VEGF) and basic fibroblast growth factor ( bFGF) in Mongolia patients with diabetic retinopathy ( DR) and to explore their roles in the progress of DR. METHODS: Eighty - three patients with diabetes were divided into three groups according to the retinopathy, no DR group ( NDR, 25 cases), background DR ( NPDR, 31 cases) and proliferative DR group (PDR, 27 cases), and 36 age - matched healthy people ( control group) were selected. Serum concentrations of VEGF and bFGF were measured using ELISA method. RESULTS: In Mongolia patients with diabetes, the serum concentrations of VEGF and bFGF were higher than those in control group; the serum concentrations of VEGF and bFGF in PDR group was higher than those of NDR and NPDR group (P CONCLUSION: High level of VEGF and bFGF may be the important pathogenic factors for DR in Mongolia patients.
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Objective:To investigate the defect types and reconstruction methods of maxillary defects. Methods:The database of 1 107 cases with maxillary defects in Peking University School and Hospital of Stomatology from January 1985 to December 2010 was established. There construction methods were re-viewed. The defect types were classified according to Brown classification system. Results: In the 1 107 cases, 1 104 cases could be classified according to Brown classification system. The most common type was 2a with 559 cases (50. 6%). Among all the 1 107 cases, 349 cases were reconstructed with auto-transplantation, 443 cases with prosthesis, 107 cases untreated, and 208 patients lost to the follow-up. There was a significant growing trend over time for the application of free flaps and a downward trend of prosthesis. The most popular free flaps were fibular flap (88 cases) and radial forearm flap (75 cases) . Rectus abdominis flap and anterolatreal thigh flap were fit for extensive maxillary defects. Conclusion:The most common defect type is 2a. Free flap has become the dominant option for maxillary reconstruc-tion. Free flaps could be selected according to the maxillary defect types.
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10.3969/j.issn.2095-4344.2013.25.020
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Effects of dietary supplementation with fructooligosaccharides on the excretion of nitrogen and phosphorus in Miichthys miiuy fries were investigated. Nine hundred Miichthys miiuy fries were divided into 3 groups, each with triplicates. The basal diet and the basal diet supplemented with carnitine groups were considered as the negative and positive controls respectively. Results showed that the nitrogen concentration in excreted feces decreased significantly in fries fed the diet supplementation with 1000 x 10(-6) fructooligosaccharides and 200 x 10(-6) carnitine (P<0.05). The ammonic-nitrogen concentration decreased significantly in the carnitine group only (P<0.05), indicating the decreasing tendency caused by the supplementation with fructooligosaccharides. Supplementation with both did not have significant effects on the concentration of phosphorus in feces of Miichthys miiuy fries.
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Animals , Administration, Oral , Dietary Supplements , Feces , Fishes , Metabolism , Nitrogen , Metabolism , Oligosaccharides , Phosphorus , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To detect gene mutations associated with autosomal dominant congenital stationary night blindness(ADCSNB) in a large Chinese family.</p><p><b>METHODS</b>Genomic DNAs were extracted from peripheral blood samples of 16 affected and 14 unaffected family members. According to 5 missense mutations in 3 genes reported previously, 4 pairs of primers were designed and corresponding exons containing the five mutation sites were amplified by polymerase chain reaction. Amplified products were purified and sequenced by MegaBACE1000 capillary array electrophoresis DNA sequencer. Full field electroretinogram (ERG, ISCEV) of patients was recorded and analyzed by Roland Consult System.</p><p><b>RESULTS</b>Dark-adapted ERG showed a-wave was normal, but b-wave of the patients was markedly decreased. None of the five missense mutations were detected in 16 affected and 14 unaffected family members.</p><p><b>CONCLUSION</b>The molecular pathogenesis of ADCSNB in this family does not involve point mutations or deletions of these five sites, which indicates the heterogeneity of ADCSNB.</p>
Subject(s)
Adult , Female , Humans , Male , Base Sequence , DNA Mutational Analysis , Molecular Sequence Data , Night Blindness , Genetics , Pedigree , Point MutationABSTRACT
Clinical teaching and studying is suffering increasing hardship.This article analysises the present condition of clinical education problems and causes,and researches whether patients are obliged to coorperate clinical teaching and studying when they protect their rights.It also researches how to practice clinical teaching and studying in educational hospital and looks for effective pathway to go out of the clinical education problem.